ABSTRACT
The diagnosis of bovine herpesvirus 5 (BoHV-5) encephalitis is confirmed after death by laboratory methods applied to brain fragments. Alternative methods to confirm ante-mortem diagnosis are important because the disease is not always lethal. The aim of this study was to investigate whether the presence of the virus genome in the cerebrospinal fluid (CSF) detected by polymerase chain reaction (PCR) might be admitted as a method for ante-mortem diagnosis. CSF samples were taken from 14 animals suffering from BoHV-5 encephalitis, diagnosed by characteristic histopathological lesions in the brain and by identification of the virus genome by PCR in different portions of the brain. Virus DNA was detected in the CSF of 21.42% (3/14) of the evaluated animals. Ante-mortem detection of the virus genome in the CSF showed low sensitivity to confirm the diagnosis. The diagnosis is confirmed by a positive result but a negative one does not discard the disease.(AU)
Subject(s)
Animals , Cattle , Cerebrospinal Fluid , Herpesvirus 5, Bovine/isolation & purification , Meningoencephalitis/diagnosis , Polymerase Chain Reaction/veterinaryABSTRACT
O objetivo deste trabalho foi determinar a prevalência das infecções latentes por BoHV-1 e por BoHV-5 em bovinos de corte criados no Estado do Paraná. Os gânglios do nervo trigêmeo foram coletados de 400 bovinos hígidos, entre 18 e 36 meses de idade, provenientes de 90 propriedades rurais localizadas em diferentes mesorregiões geográficas do Estado e abatidos em frigorífico com Serviço de Inspeção Federal. A reação em cadeia da polimerase com amplificação do gene que codifica a glicoproteína C foi empregada para a detecção do DNA viral. Cento e nove bovinos eram herpéticos (27,25%), sendo 14,25% (57/400) infectados com BoHV-1, 9,75% (39/400) infectados com BoHV-5 e 3,25% (13/400) portadores de infecção mista. A distribuição geográfica foi heterogênea e as infecções foram mais prevalentes nas mesorregiões localizadas ao norte do Estado. A vigilância para a encefalite por BoHV-5 deve ser intensificada na mesorregião Noroeste.
The prevalence of latent infection with BoHV-1 or BoHV-5 in beef cattle raised in the state of Paraná, Brazil, was studied. The trigeminal ganglia were collected in a slaughterhouse from 400 healthy cattle, 18 to 36 months old, raised in 90 farms located in distinct geographical regions of the state. Polymerase chain reaction for amplification of the gene encoding C glycoprotein was performed to detect virus DNA. One hundred and nine (27.25%) animals were herpetic; 14.25% (57/400) were infected with BoHV-1, 9.75% (39/400) were infected with BoHV-5 and 3.25% (13/400) had mixed infection. The geographical distribution was heterogeneous and the infections were more prevalent in the north of the state. The surveillance for BoHV-5 encephalitis should be intensive in the Northwest region.
Subject(s)
Animals , Cattle , Epidemiology , Herpesvirus 1, Bovine , Encephalitis, Herpes Simplex/veterinary , Encephalitis/veterinary , Diagnostic Techniques and Procedures/veterinaryABSTRACT
Utilizaram-se os modelos lineares generalizados com as funções de ligação probit e logit na avaliação da prenhez precoce, e observaram-se os efeitos na variabilidade genética e na seleção de reprodutores quando diferentes idades são adotadas na definição dessa característica. A prenhez precoce foi estudada aos 15 (PP15) e 21 meses (PP21). Correlações entre os valores genéticos preditos e a porcentagem de touros em comum, considerando 10% dos touros com maiores valores genéticos (TOP10), entre a classificação dos modelos com função de ligação logit e probit e em cada modelo entre PP15 e PP21, foram calculadas. As herdabilidades para PP15 e PP21 foram próximas entre os modelos, exceto para PP15 utilizando a função de ligação probit. Aquelas entre os modelos e a TOP/10 foram altas. Os critérios de Akaike e Bayesiano apresentaram-se semelhantes entre os modelos. As correlações entre PP15 e PP21 e a TOP10, considerando o mesmo modelo, foram de média magnitude.
This study aimed to use the generalized linear models with probit and logit link function to evaluate early pregnancy, and to observe the effects on genetic variability and on sire selection when different ages are adopted in the definition of this trait. Early pregnancy was studied at 15 (EP15), and 21 (EP21) months. The analysis was done in R software. Pearson correlations (PC), between genetic predicted values and percentage of bulls in common considering only 10% of bulls with higher genetic values (TOP 10), between classification by logit and probit models and in each model among EP15 and EP21, were calculated. The heritability for EP15 and EP21 were close between models, except for EP15 using probit link function. PC and TOP10 among models were high. The Akaike and Bayesian criteria reported was similar between models. TOP10, considering the same model, among EP15-EP21 were moderated between EP15-EP21.